Emmanuel Eric Pazo

← Publications

The impact of hydroxybutyl chitosan and 5-fluorouracil on the procedure of endoscopic endonasal dacryocystorhinostomy

Jia Qi Lin, Jin Zhao, Xun Liu, Emmanuel Eric Pazo, Qing Wang, Li Zhu, T.‐S. LIN

Indian Journal of Ophthalmology, 2024 · article · doi:10.4103/ijo.ijo_3375_23 · cited by 2 · Open access (CC BY-NC-SA)

Other

Abstract

PURPOSE: To investigate the effects of hydroxybutyl chitosan (HBCS) with and without 5-fluorouracil (5-FU) during endoscopic endonasal dacryocystorhinostomy (Endo-DCR). In addition, the present study observed the impact of HBCS and 5-FU on the functions of the nasal mucosal cell population in vivo . METHODS: Patients were randomized into HBCS (group A), HBCS combined with 5-FU (group B), and gelatin sponge control group (group C). 16S rRNA high-throughput sequencing technology examined the conjunctival sac and nasal flora changes. In addition, CCK8, cell scratching, and flow cytometry were used to investigate the effects of HBCS and 5-FU on the nasal mucosal cell populations. RESULTS: Subjects in groups A, B, and C had anastomotic areas of 21.83 ± 12.69 mm 2 , 21.57 ± 14.53 mm 2 , and 12.45 ± 8.16 mm 2 , respectively ( P = 0.0359). Group A had less severe epiphora than the other two groups at 1-, 2-, and 12-week postoperative follow-up ( P < 0.05). Complications around the anastomosis in group A were the least severe of the three groups ( P = 0.0259). After surgery, the proportion of pathogenic bacteria in the conjunctival sac and nasal cavity was higher in groups A and B than in healthy adults. At the 2-week follow-up, the structure of nasal flora in group A was more similar to that of the healthy adults compared to group B. CONCLUSION: Intraoperative use of HBCS at the anastomose improves the postoperative outcome of En-DCR. 5-FU cannot give better postoperative results in En-DCR and is detrimental to the normalization of the postoperative flora in patients with chronic dacryocystitis. At the cellular level, both HBCS and 5-FU inhibit the migration of nasal mucosal cell populations, and 5-FU inhibits proliferation but does not promote apoptosis.

Keywords: hydroxybutyl chitosan; 5-fluorouracil; endoscopic endonasal dacryocystorhinostomy; chronic dacryocystitis; nasal mucosa; nasal microbiota; anastomotic healing; epiphora

Access

How to cite

Lin, J. Q., Zhao, J., Liu, X., Pazo, E. E., Wang, Q., Zhu, L., LIN, T. (2024). The impact of hydroxybutyl chitosan and 5-fluorouracil on the procedure of endoscopic endonasal dacryocystorhinostomy. Indian Journal of Ophthalmology. https://doi.org/10.4103/ijo.ijo_3375_23

@article{lin2024the,
  title = {{The impact of hydroxybutyl chitosan and 5-fluorouracil on the procedure of endoscopic endonasal dacryocystorhinostomy}},
  author = {Lin, Jia Qi and Zhao, Jin and Liu, Xun and Pazo, Emmanuel Eric and Wang, Qing and Zhu, Li and LIN, T.‐S.},
  journal = {Indian Journal of Ophthalmology},
  year = {2024},
  doi = {10.4103/ijo.ijo_3375_23},
  url = {https://doi.org/10.4103/ijo.ijo_3375_23},
}

Related work