Emmanuel Eric Pazo

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No side effects on retina or vitreous microenvironment by YAG laser vitreolysis in vivo

Tiezhu Lin, Mingqin Zhang, Bing Heng Wu, Yuanlong An, Emmanuel Eric Pazo, Rong Li, Lijun Shen

Research Square, 2023 · preprint · doi:10.21203/rs.3.rs-3272066/v1 · cited by 2 · Open access (CC BY)

Retina & vascular imaging

Abstract

Abstract Background: To explore the safety of yttrium-aluminum-garnet (YAG) laser vitreolysis based on the histological examination of the retina and the alteration of vitreous cytokines in vivo. Methods: Nine male New Zealand rabbits underwent YAG laser vitreolysis of 10 mJ x 500 pulses in the left eyes, while the right eyes were used as controls. Intraocular pressure, color fundus photography, and ultrasound B scan were measured before, as well as 1 day, 4 weeks, and 12 weeks after YAG laser vitreolysis. Three rabbits were euthanized 1 day, 4 weeks, and 12 weeks after treatment, respectively. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and hematoxylin-eosin (H&E) staining were used to look for pathological changes in the retina. An enzyme-linked immunosorbent assay (ELISA) was utilized to detect the expression of vascular endothelial growth factor (VEGF) and some inflammatory cytokines, including interferon inducible protein 10 (IP-10), monocyte chemoattractant protein 1 (MCP-1) and interlenkin 6 (IL-6) in the vitreous humor. The ascorbic acid (AsA) and total reactive antioxidant potential (TRAP) in the vitreous humor were also measured. Results: Following YAG laser vitreolysis, the levels of VEGF, IP-10, MCP-1, IL6, AsA, and TRAP in the vitreous humor did not change substantially (P > 0.05). There were no detectable pathological changes in the retinal tissues, and no apoptotic signal was found. Conclusions: YAG laser vitreolysis has no noticeable effects on retinal tissue or the microenvironment of the vitreous, so it is a safe procedure.

Keywords: retinal safety; vitreous humor; retinal histology; apoptosis; vascular endothelial growth factor; inflammatory cytokines; oxidative stress; vitamin C

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How to cite

Lin, T., Zhang, M., Wu, B. H., An, Y., Pazo, E. E., Li, R., Shen, L. (2023). No side effects on retina or vitreous microenvironment by YAG laser vitreolysis in vivo. Research Square. https://doi.org/10.21203/rs.3.rs-3272066/v1

@article{lin2023no,
  title = {{No side effects on retina or vitreous microenvironment by YAG laser vitreolysis in vivo}},
  author = {Lin, Tiezhu and Zhang, Mingqin and Wu, Bing Heng and An, Yuanlong and Pazo, Emmanuel Eric and Li, Rong and Shen, Lijun},
  journal = {Research Square},
  year = {2023},
  doi = {10.21203/rs.3.rs-3272066/v1},
  url = {https://doi.org/10.21203/rs.3.rs-3272066/v1},
}

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